Fermentas t4 ligase protocol

Fermentas t4 ligase protocol

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PRODUCT INFORMATION. T4 DNA Ligase, _ u/µl. #___. __. Lot __. Expiry Date __. Store at -20°C bb www.thermoscientific.com/onebio. Ordering Information.

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Aug 23, 2002 - Fermentas, ISO9002 DNA Insert Ligation into Vector DNA (with T4 DNA Ligase). In a microcentrifuge tube prepare 5-10µl mix in water or TE Note: T4 DNA Ligase is unstable on ice for long periods. Therefore, Invitrogen T4 DNA Ligase can be used to join DNA fragments with staggered or blunt ends. Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or

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The T4 DNA Ligase Buffer should be thawed and resuspended at room temperature. Gently mix the reaction by pipetting up and down and microfuge briefly. Jun 14, 2006 - In total 2vials. Description. T4 DNA Ligase catalyzes the formation of a phosphodi- Activity in Fermentas REase Buffers*, %. (in comparison to T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5?-phosphate and the 3?-hydroxyl groups of adjacent nucleotides in either a Ligase independent cloning (LIC) is a simple, fast and relatively cheap method to produce expression In the following protocol 600 ng BsaI-digested LIC vector is treated with T4 DNA polymerase to produce .. Fermentas (EP0502). T4 DNAJul 9, 2012 - I proceeded to ligation, I used T4 DNA ligase, NEB nad 10X NEB buffer. . 10 ul 6x-loading dye (Fermentas) (or another suitable DNA loanding Enzyme Information: T4 DNA Ligase. Product name, : T4 DNA Ligase. Cat#, : EL0015. Unit size, : 200u. EC#, : 6.5.1.1. CAS#, : 9015-85-4. Concentration, : 1u/µl.

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